IHC-plus(tm) MSN / Moesin Antibody

LifeSpan BioSciences

IHC-plus(tm) MSN / Moesin Antibody

23

Immunohistochemistry: LS-B2180 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2180 was determined to be 5 ug/ml. Immunoprecipitation: Denatured only. 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml for 2 hours at RT. Positive control: Raji cells, placenta.. ELISA, IF, IHC, IHC-P (5 µg/ml), IP, WB (1 - 2 µg/ml) Immunohistochemistry: LS-B2180 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2180 was determined to be 5 ug/ml. Immunoprecipitation: Denatured only. 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml for 2 hours at RT. Positive control: Raji cells, placenta.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.2 mg/ml

Unconjugated

MSN

moesin

HEL70, IMD50, moesin, epididymis luminal protein 70, membrane-organizing extension spike protein

Mouse

IgG1

Bovine, Human, Rat

-20°C,2°C to 8°C

41116161