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ProductsBack/IHC-plus(tm) MTOR Antibody

IHC-plus(tm) MTOR Antibody

LS-B1354
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMTOR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MTOR Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    TOR antibody can be used for the detection of TOR by Western blot at 1 to 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Western Blot:. ELISA, ICC, IHC, IHC-P (5 µg/ml), WB (1 µg/ml) TOR antibody can be used for the detection of TOR by Western blot at 1 to 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Western Blot:
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2475
  • Target name
    MTOR
  • Target description
    mechanistic target of rapamycin kinase
  • Target synonyms
    FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS, serine/threonine-protein kinase mTOR, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated protein 1, mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), rapamycin and FKBP12 target 1, rapamycin associated protein FRAP2, rapamycin target protein 1, tyrosine-protein kinase mTOR
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MTOR using anti-MTOR antibody (A00003-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Jurkat whole cell ltsates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified polyclonal antibody (Catalog # A00003-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
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