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Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with mTOR/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with mTOR/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with mTOR/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4°C, DAB staining.

MTOR Polyclonal Antibody

BS-1992R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMTOR
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Overview

  • Supplier
    Bioss
  • Product Name
    mTOR/FRAP Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2475
  • Target name
    MTOR
  • Target description
    mechanistic target of rapamycin kinase
  • Target synonyms
    FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FRAP; FRAP1; FRAP2; mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR; SKS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42345
  • Protein Name
    Serine/threonine-protein kinase mTOR
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Targeting the miR-122/PKM2 autophagy axis relieves arsenic stress. Wang Y et al., 2020 Feb 5, J Hazard Mater
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  • miR-496 remedies hypoxia reoxygenation-induced H9c2 cardiomyocyte apoptosis via Hook3-targeted PI3k/Akt/mTOR signaling pathway activation. Jin Y et al., 2020 Jan, J Cell Biochem
    Read more
  • AMPK mediates the neurotoxicity of iron oxide nanoparticles retained in mitochondria or lysosomes. Huang H et al., 2019 Jul 17, Metallomics
    Read more
  • Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis. Wang YF et al., 2018 Mar 28, Aging (Albany NY)
    Read more
  • Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica). Li X et al., 2017 Dec, J Therm Biol
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  • Synergistic effect of copper and arsenic upon oxidative stress, inflammation and autophagy alterations in brain tissues of Gallus gallus. Sun X et al., 2018 Jan, J Inorg Biochem
    Read more
  • Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma. Tian J et al., 2017 Mar, Oncol Lett
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  • Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats. Zou D et al., 2016 Jul 1, Life Sci
    Read more
  • Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo. Madka V et al., 2016 Jan, Cancer Prev Res (Phila)
    Read more