Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with mTOR/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with mTOR/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4°C, DAB staining.
MTOR Polyclonal Antibody
BS-1992R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMTOR
Overview
- SupplierBioss
- Product NameMTOR Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2475
- Target nameMTOR
- Target descriptionmechanistic target of rapamycin kinase
- Target synonymsFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS, serine/threonine-protein kinase mTOR, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated protein 1, mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), rapamycin and FKBP12 target 1, rapamycin associated protein FRAP2, rapamycin target protein 1, tyrosine-protein kinase mTOR
- HostRabbit
- IsotypeIgG
- Protein IDP42345
- Protein NameSerine/threonine-protein kinase mTOR
- Storage Instruction-20°C
- UNSPSC12352203
References
- Wang Y, Zhao H, Guo M, et al. Targeting the miR-122/PKM2 autophagy axis relieves arsenic stress. J Hazard Mater. 2020,383:121217. doi: 10.1016/j.jhazmat.2019.121217Read this paper
- Jin Y, Ni S. miR-496 remedies hypoxia reoxygenation-induced H9c2 cardiomyocyte apoptosis via Hook3-targeted PI3k/Akt/mTOR signaling pathway activation. J Cell Biochem. 2020,121(1):698-712. doi: 10.1002/jcb.29316Read this paper
- Huang H, Zhou M, Ruan L, et al. AMPK mediates the neurotoxicity of iron oxide nanoparticles retained in mitochondria or lysosomes. Metallomics. 2019,11(7):1200-1206. doi: 10.1039/c9mt00103dRead this paper
- Wang YF, Sun XF, Han ZL, et al. Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis. Aging (Albany NY). 2018,10(3):463-480. doi: 10.18632/aging.101405Read this paper
- Li X, Qiu J, Liu H, et al. Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica). J Therm Biol. 2017,70(Pt B):37-45. doi: 10.1016/j.jtherbio.2017.10.008Read this paper
- Sun X, Li J, Zhao H, et al. Synergistic effect of copper and arsenic upon oxidative stress, inflammation and autophagy alterations in brain tissues of Gallus gallus. J Inorg Biochem. 2018,178:54-62. doi: 10.1016/j.jinorgbio.2017.10.006Read this paper
- Tian J, Liu R, Qu Q. Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma. Oncol Lett. 2017,13(3):1437-1443. doi: 10.3892/ol.2017.5580Read this paper
- Zou D, Geng N, Chen Y, et al. Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats. Life Sci. 2016,156:7-14. doi: 10.1016/j.lfs.2016.05.026Read this paper
- Madka V, Mohammed A, Li Q, et al. Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo. Cancer Prev Res (Phila). 2016,9(1):53-62. doi: 10.1158/1940-6207.CAPR-15-0199Read this paper
Datasheet
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