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mTOR antibody

20R-2063
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    mTOR antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MTOR using anti-MTOR antibody (A00003-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Jurkat whole cell ltsates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified polyclonal antibody (Catalog # A00003-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
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Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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