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mTOR antibody

20R-2063
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    mTOR antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTOR using anti-MTOR antibody (A00003-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Jurkat whole cell ltsates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified polyclonal antibody (Catalog # A00003-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
Product group Antibodies
Boster Bio
Anti-mTOR/MTOR Antibody Picoband(r)A00003-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetMTOR
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Product group Antibodies
Assay Genie
Phospho-mTOR-S2448 Monoclonal AntibodyCABP0115
ReactivityHuman
TargetMTOR
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Product group Antibodies
Biomatik
Serine/threonine-protein kinase mTOR (mTOR) Polyclonal AntibodyCAU25274
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityPorcine
TargetMTOR
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Product group Antibodies
Everest Biotech
Goat anti-MTOR / FRAP2 AntibodyEB11384
ApplicationsImmunoFluorescence, ELISA
ReactivityCanine, Human, Mouse, Porcine, Rat
TargetMTOR
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Product group Antibodies
RayBiotech
Anti-P-mTor Antibody130-10612
ApplicationsELISA
ReactivityHuman
TargetMTOR
  • SizePrice
Product group Antibodies
References
GeneTex
mTOR antibody [C3], C-termGTX101557
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMTOR
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with mTOR/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
References
Bioss
MTOR Polyclonal AntibodyBS-1992R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Goat, Human, Mouse, Rabbit, Rat, Sheep
TargetMTOR
  • SizePrice
Anti-mTOR Antibody
Product group Antibodies
Antibodies.com
Anti-mTOR AntibodyA121172
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
  • SizePrice
Western blot analysis in human cell line K562.
Product group Antibodies
Atlas Antibodies
Anti-MTOR AntibodyAMAB91508
ApplicationsWestern Blot
ReactivityHuman
TargetMTOR
  • SizePrice
Western Blot analysis of HuvEc cells using mTOR Polyclonal Antibody
Product group Antibodies
Cusabio
MTOR AntibodyCSB-PA003333
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMTOR
  • SizePrice