IHC-plus(tm) MYL2 Antibody (clone AT3B2)

LifeSpan BioSciences

IHC-plus(tm) MYL2 Antibody (clone AT3B2)

23

Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.. ELISA, IHC, IHC-P (5 µg/ml), WB (1:1000 - 1:2000) Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

AT3B2

1 mg/ml

Unconjugated

MYL2

myosin light chain 2

CMH10, MFM12, MLC-2, MLC-2s/v, MLC-2v, MLC2, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, RLC of myosin, S15D myosin regulatory light chain 2, T160D myosin regulatory light chain 2, cardiac myosin light chain 2, cardiac ventricular myosin light chain 2, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, slow cardiac myosin regulatory light chain 2, ventricular myosin light chain 2

Mouse

IgG2b

Human, Mouse, Rat

-20°C

41116161