IHC-plus(tm) NANOG Antibody (aa291-304)

LifeSpan BioSciences

IHC-plus(tm) NANOG Antibody (aa291-304)

23

Immunohistochemistry: LS-B3017 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3017 was determined to be 2 ug/ml.. ELISA (5 - 10 µg/ml), IHC, IHC-P (2 µg/ml), Peptide-ELISA (1:128000), WB (0.05 - 0.1 µg/ml) Immunohistochemistry: LS-B3017 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3017 was determined to be 2 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

NANOG

Nanog homeobox

homeobox protein NANOG, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Goat

Human

-20°C

41116161