IHC-plus(tm) NEK2 Antibody

LifeSpan BioSciences

IHC-plus(tm) NEK2 Antibody

23

Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.. ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.1 mg/ml

Unconjugated

NEK2

NIMA related kinase 2

HsPK21; NEK2A; NIMA (never in mitosis gene a)-related kinase 2; nimA-like protein kinase 1; nimA-related protein kinase 2; NLK1; PPP1R111; protein phosphatase 1, regulatory subunit 111; RP67; serine/threonine-protein kinase Nek2

Rabbit

IgG

Human

-20°C

12352203