IHC-plus(tm) NEK2 Antibody

LifeSpan BioSciences

IHC-plus(tm) NEK2 Antibody

23

Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.. ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.1 mg/ml

Unconjugated

...

NEK2

NIMA related kinase 2

HsPK21, NEK2A, NLK1, PPP1R111, RP67, serine/threonine-protein kinase Nek2, NIMA (never in mitosis gene a)-related kinase 2, nimA-like protein kinase 1, nimA-related protein kinase 2, protein phosphatase 1, regulatory subunit 111

Rabbit

IgG

Human

-20°C

12352203