NEK2 antibody detects NEK2 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. NEK2 antibody (GTX118735) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
NEK2 antibody detects NEK2 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. NEK2 antibody (GTX118735) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
NEK2 antibody
GTX118735
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNEK2
Overview
- SupplierGeneTex
- Product NameNEK2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.47 mg/ml
- ConjugateUnconjugated
- Gene ID4751
- Target nameNEK2
- Target descriptionNIMA related kinase 2
- Target synonymsHsPK21, NEK2A, NLK1, PPP1R111, RP67, serine/threonine-protein kinase Nek2, NIMA (never in mitosis gene a)-related kinase 2, nimA-like protein kinase 1, nimA-related protein kinase 2, protein phosphatase 1, regulatory subunit 111
- HostRabbit
- IsotypeIgG
- Protein IDP51955
- Protein NameSerine/threonine-protein kinase Nek2
- Scientific DescriptionProtein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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