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ProductsBack/IHC-plus(tm) NGFRAP1 / NADE / Bex Antibody

IHC-plus(tm) NGFRAP1 / NADE / Bex Antibody

LS-B532
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetBEX3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NGFRAP1 / NADE / Bex Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    NADE antibody can be used for detection of NADE by Western blot at 1 ug/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml) NADE antibody can be used for detection of NADE by Western blot at 1 ug/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27018
  • Target name
    BEX3
  • Target description
    brain expressed X-linked 3
  • Target synonyms
    Bex, DXS6984E, HGR74, Hero20, NADE, NGFRAP1, protein BEX3, brain-expressed X-linked protein 3, nerve growth factor receptor (TNFRSF16) associated protein 1, ovarian granulosa cell 13.0 kDa protein HGR74, p75NTR-associated cell death executor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BEX3 using anti-BEX3 antibody (A09675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BEX3 antigen affinity purified polyclonal antibody (Catalog # A09675-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BEX3 at approximately 28 kDa. The expected band size for BEX3 is at 28 kDa.
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