IHC-plus(tm) NOS3 / eNOS Antibody

LifeSpan BioSciences

IHC-plus(tm) NOS3 / eNOS Antibody

23

Immunohistochemistry: LS-B3674 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3674 was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml), WB Immunohistochemistry: LS-B3674 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3674 was determined to be 10 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

NOS3

nitric oxide synthase 3

ECNOS, eNOS, nitric oxide synthase 3, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, nitric oxide synthase 3 (endothelial cell)

Rabbit

Human, Mouse, Rat

-20°C

41116161