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ProductsBack/IHC-plus(tm) NR1D1 Antibody (clone 4F6)

IHC-plus(tm) NR1D1 Antibody (clone 4F6)

LS-B4487
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetNR1D1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NR1D1 Antibody (clone 4F6)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Cell lysate) - positive control NIH/3T3. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).. ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), RNAi, WB Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Cell lysate) - positive control NIH/3T3. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4F6
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9572
  • Target name
    NR1D1
  • Target description
    nuclear receptor subfamily 1 group D member 1
  • Target synonyms
    EAR1, REVERBA, REVERBalpha, THRA1, THRAL, ear-1, hRev, nuclear receptor subfamily 1 group D member 1, Rev-ErbAalpha, V-erbA-related protein 1, nuclear receptor Rev-ErbA-alpha, rev-erbA-alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of KDM1B using anti-KDM1B antibody (M01077-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM1B antigen affinity purified monoclonal antibody (Catalog # M01077-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM1B at approximately 55 kDa. The expected band size for KDM1B is at 67 kDa.
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