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ProductsBack/IHC-plus(tm) NSD3 / WHSC1L1 Antibody

IHC-plus(tm) NSD3 / WHSC1L1 Antibody

LS-B13871
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNSD3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NSD3 / WHSC1L1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 72kDa/154kDa/156kDa/160kDa/161kDa, while the observed MW by Western blot was 85kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:200 - 1:2000) The predicted MW is 72kDa/154kDa/156kDa/160kDa/161kDa, while the observed MW by Western blot was 85kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54904
  • Target name
    NSD3
  • Target description
    nuclear receptor binding SET domain protein 3
  • Target synonyms
    KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328, histone-lysine N-methyltransferase NSD3, Wolf-Hirschhorn syndrome candidate 1-like 1, nuclear SET domain-containing protein 3, protein whistle
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NSD3 using anti-NSD3 antibody (A32443-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSD3 antigen affinity purified polyclonal antibody (Catalog # A32443-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSD3 at approximately 90 kDa. The expected band size for NSD3 is at 90 kDa.
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