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ProductsBack/IHC-plus(tm) OGT / O-GLCNAC Antibody

IHC-plus(tm) OGT / O-GLCNAC Antibody

LS-B13864
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetOGT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) OGT / O-GLCNAC Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 74kDa/103kDa/115kDa/116kDa, while the observed MW by Western blot was 118kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:100), IP (1:20 - 1:50), WB (1:500 - 1:2000) The predicted MW is 74kDa/103kDa/115kDa/116kDa, while the observed MW by Western blot was 118kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8473
  • Target name
    OGT
  • Target description
    O-linked N-acetylglucosamine (GlcNAc) transferase
  • Target synonyms
    HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), O-linked N-acetylglucosamine transferase 110 kDa subunit, UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of OGT using anti-OGT antibody (PB9767). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGT antigen affinity purified polyclonal antibody (Catalog # PB9767) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGT at approximately 117KD. The expected band size for OGT is at 117KD.
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