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ProductsBack/IHC-plus(tm) PCNA Antibody (clone ABT-PCNA)

IHC-plus(tm) PCNA Antibody (clone ABT-PCNA)

LS-B16161
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPCNA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PCNA Antibody (clone ABT-PCNA)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Colon carcinoma. Resulting localization is Nuclear
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:200 - 1:400), IHC-P (1:50), WB (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Colon carcinoma. Resulting localization is Nuclear
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABT-PCNA
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5111
  • Target name
    PCNA
  • Target description
    proliferating cell nuclear antigen
  • Target synonyms
    ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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