IHC-plus(tm) PCNA Antibody (clone PC10)

LifeSpan BioSciences

IHC-plus(tm) PCNA Antibody (clone PC10)

23

Immunohistochemistry: LS-B3434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3434 was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml), IP, WB (1:5000) Immunohistochemistry: LS-B3434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3434 was determined to be 10 ug/ml.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

PC10

1 mg/ml

Unconjugated

PCNA

proliferating cell nuclear antigen

ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin

Mouse

Chicken, Drosophila, Human, Mouse, Primate, Rat, Yeast, Zebra Fish

-20°C,2°C to 8°C

41116161