IHC-plus(tm) PDI / P4HB Antibody

LifeSpan BioSciences

IHC-plus(tm) PDI / P4HB Antibody

23

Immunohistochemistry: LS-B2372 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2372 was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.. ICC (1:50), IF (1:50), IHC, IHC-P (1:50), WB (1:1000) Immunohistochemistry: LS-B2372 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2372 was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

P4HB

prolyl 4-hydroxylase subunit beta

CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, protein disulfide-isomerase, cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, p55, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide, prolyl 4-hydroxylase, beta polypeptide, protein disulfide isomerase family A, member 1, protein disulfide isomerase-associated 1, protein disulfide isomerase/oxidoreductase, protocollagen hydroxylase, testicular secretory protein Li 32, thyroid hormone-binding protein p55

Rabbit

IgG

Human

-20°C,2°C to 8°C

41116161