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ProductsBack/IHC-plus(tm) PLTP Antibody (aa470-490)

IHC-plus(tm) PLTP Antibody (aa470-490)

LS-B12104
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPLTP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PLTP Antibody (aa470-490)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, immunofluorescence/immunocytochemistry and immunohistochemistry. This antibody does not exhibit neutralizing activity.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:50 - 1:100), IHC, IHC-Fr (1:200), IHC-P (1:100), WB (1:500) Suitable for use in Western Blot, immunofluorescence/immunocytochemistry and immunohistochemistry. This antibody does not exhibit neutralizing activity.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5360
  • Target name
    PLTP
  • Target description
    phospholipid transfer protein
  • Target synonyms
    BPIFE, HDLCQ9, phospholipid transfer protein, BPI fold containing family E, lipid transfer protein II
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PLTP using anti-PLTP antibody (A02209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLTP antigen affinity purified polyclonal antibody (Catalog # A02209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLTP at approximately 65KD. The expected band size for PLTP is at 65KD.
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