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ProductsBack/IHC-plus(tm) PPP1CA / PP1-Alpha Antibody

IHC-plus(tm) PPP1CA / PP1-Alpha Antibody

LS-B13867
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPPP1CA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PPP1CA / PP1-Alpha Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 32kDa/37kDa/38kDa, while the observed MW by Western blot was 38kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 32kDa/37kDa/38kDa, while the observed MW by Western blot was 38kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5499
  • Target name
    PPP1CA
  • Target description
    protein phosphatase 1 catalytic subunit alpha
  • Target synonyms
    PP-1A, PP1A, PP1alpha, PPP1A, serine/threonine-protein phosphatase PP1-alpha catalytic subunit, protein phosphatase 1, catalytic subunit, alpha isozyme, serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit, testicular tissue protein Li 155
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PPP1CA + PPP1CB using anti-PPP1CA + PPP1CB antibody (M02801). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1CA + PPP1CB antigen affinity purified monoclonal antibody (Catalog # M02801) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1CA + PPP1CB at approximately 38 kDa. The expected band size for PPP1CA + PPP1CB is at 38 kDa.
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