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ProductsBack/IHC-plus(tm) PRDX2 / Peroxiredoxin 2 Antibody

IHC-plus(tm) PRDX2 / Peroxiredoxin 2 Antibody

LS-B14874
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPRDX2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PRDX2 / Peroxiredoxin 2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 15kDa/21kDa, while the observed MW by Western blot was 20kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 15kDa/21kDa, while the observed MW by Western blot was 20kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7001
  • Target name
    PRDX2
  • Target description
    peroxiredoxin 2
  • Target synonyms
    HEL-S-2a, NKEF-B, NKEFB, PRP, PRX2, PRXII, PTX1, TDPX1, TPX1, TSA, peroxiredoxin-2, epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1, thioredoxin-dependent peroxide reductase 1, thioredoxin-dependent peroxiredoxin 2, torin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Peroxiredoxin 2/PRDX2 using anti-Peroxiredoxin 2/PRDX2 antibody (A01982-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 2/PRDX2 antigen affinity purified polyclonal antibody (Catalog # A01982-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 2/PRDX2 at approximately 22 kDa. The expected band size for Peroxiredoxin 2/PRDX2 is at 22 kDa.
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