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ProductsBack/IHC-plus(tm) PTPRJ / CD148 Antibody

IHC-plus(tm) PTPRJ / CD148 Antibody

LS-B15887
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPTPRJ
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PTPRJ / CD148 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    The predicted MW is 57kDa/145kDa, while the observed MW by Western blot was 260kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:200), WB (1:200 - 1:1000) The predicted MW is 57kDa/145kDa, while the observed MW by Western blot was 260kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5795
  • Target name
    PTPRJ
  • Target description
    protein tyrosine phosphatase receptor type J
  • Target synonyms
    CD148, DEP1, HPTP eta, HPTPeta, R-PTP-ETA, R-PTP-J, SCC1, THC10, receptor-type tyrosine-protein phosphatase eta, CD148 antigen, density-enhanced phosphatase 1, human density enhanced phosphatase-1, protein tyrosine phosphatase, receptor type, J polypeptide, protein-tyrosine phosphatase eta, susceptibility to colon cancer 1, mouse, homolog of
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEP1/PTPRJ antigen affinity purified polyclonal antibody (Catalog # A02319-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DEP1/PTPRJ at approximately 240 kDa. The expected band size for DEP1/PTPRJ is at 146 kDa.
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