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IHC-plus(tm) PTRF / CAVIN Antibody

LS-B10088
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCAVIN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PTRF / CAVIN Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 43 kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml) PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 43 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID284119
  • Target name
    CAVIN1
  • Target description
    caveolae associated protein 1
  • Target synonyms
    CAVIN, CGL4, FKSG13, PTRF, cavin-1, caveolae-associated protein 1, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12, congenital generalized lipodystrophy 4, polymerase I and transcript release factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTRF/CAVIN1 antigen affinity purified polyclonal antibody (Catalog # A31732-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTRF/CAVIN1 at approximately 50-55 kDa. The expected band size for PTRF/CAVIN1 is at 43 kDa.
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