IHC-plus(tm) ROBO1 Antibody (aa994-1006)
LS-B3011
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rat, Xenopus
TargetROBO1
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) ROBO1 Antibody (aa994-1006)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B3011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3011 was determined to be 2 ug/ml.. IF (10 µg/ml), IHC, IHC-P (2 µg/ml), Peptide-ELISA (1:8000), WB (1 µg/ml) Immunohistochemistry: LS-B3011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3011 was determined to be 2 ug/ml.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID6091
- Target nameROBO1
- Target descriptionroundabout guidance receptor 1
- Target synonymsCPHD8, DUTT1, NORS, NYS8, SAX3, roundabout homolog 1, deleted in U twenty twenty, roundabout, axon guidance receptor, homolog 1
- HostGoat
- ReactivityBovine, Canine, Chicken, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rat, Xenopus
- Storage Instruction-20°C
- UNSPSC41116161


![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [C2C3], C-term (GTX113826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113826/GTX113826_41066_20191004_WB_TPM_watermark_w_23060501_823.webp)




