IHC-plus(tm) SKP2 Antibody

LifeSpan BioSciences

IHC-plus(tm) SKP2 Antibody

23

Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.

Immunohistochemistry: LS-B2487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2487 was determined to be 5 ug/ml. Positive control: HeLa, Jurkat and 293T cell lines.. ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 µg/ml), IP, WB (1 - 2 µg/ml) Immunohistochemistry: LS-B2487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2487 was determined to be 5 ug/ml. Positive control: HeLa, Jurkat and 293T cell lines.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.5 mg/ml

Unconjugated

...

SKP2

S-phase kinase associated protein 2

CDK2/cyclin A-associated protein p45; FBL1; F-box/LRR-repeat protein 1; FBXL1; FLB1; p45; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2, E3 ubiquitin protein ligase

Mouse

IgG1

Human

-20°C,2°C to 8°C

12352203