IHC-plus(tm) SMURF1 Antibody (aa66-96)

LifeSpan BioSciences

IHC-plus(tm) SMURF1 Antibody (aa66-96)

23

Immunohistochemistry: LS-B3450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3450 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not been evaluated for use in IHC with mouse samples.. IHC, IHC-P (10 µg/ml), WB (1:1000) Immunohistochemistry: LS-B3450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3450 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not been evaluated for use in IHC with mouse samples.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

2 mg/ml

Unconjugated

SMURF1

SMAD specific E3 ubiquitin protein ligase 1

E3 ubiquitin-protein ligase SMURF1, E3 ubiquitin ligase SMURF1, HECT-type E3 ubiquitin transferase SMURF1, Smad ubiquitination regulatory factor 1, Smad-specific E3 ubiquitin ligase 1, hSMURF1

Rabbit

Human, Mouse

-20°C,2°C to 8°C

41116161