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ProductsBack/IHC-plus(tm) SMURF1 Antibody (aa66-96)

IHC-plus(tm) SMURF1 Antibody (aa66-96)

LS-B3450
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSMURF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SMURF1 Antibody (aa66-96)
  • Delivery Days Customer
    23
  • Antibody Specificity
    This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.
  • Application Supplier Note
    Immunohistochemistry: LS-B3450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3450 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not been evaluated for use in IHC with mouse samples.. IHC, IHC-P (10 µg/ml), WB (1:1000) Immunohistochemistry: LS-B3450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3450 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not been evaluated for use in IHC with mouse samples.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57154
  • Target name
    SMURF1
  • Target description
    SMAD specific E3 ubiquitin protein ligase 1
  • Target synonyms
    E3 ubiquitin ligase SMURF1; E3 ubiquitin-protein ligase SMURF1; HECT-type E3 ubiquitin transferase SMURF1; hSMURF1; Smad ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203