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ProductsBack/IHC-plus(tm) SNAI2 / SLUG Antibody

IHC-plus(tm) SNAI2 / SLUG Antibody

LS-B554
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSNAI2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SNAI2 / SLUG Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Slug antibody can be used for detection of Slug by Western blot at 0.5 ug/mL. Antibody can also be used for immunofluorescence starting at 20 ug/mL. Antibody validated: Western Blot in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 29 kDa Observed: 30 kDa. ELISA, IF, IHC, IHC-P (2.5 µg/ml), WB (1 - 2 µg/ml) Slug antibody can be used for detection of Slug by Western blot at 0.5 ug/mL. Antibody can also be used for immunofluorescence starting at 20 ug/mL. Antibody validated: Western Blot in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 29 kDa Observed: 30 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6591
  • Target name
    SNAI2
  • Target description
    snail family transcriptional repressor 2
  • Target synonyms
    SLUG, SLUGH, SLUGH1, SNAIL2, WS2D, zinc finger protein SNAI2, neural crest transcription factor SLUG, protein snail homolog 2, slug (chicken homolog), zinc finger protein, snail family zinc finger 2, snail homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.
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