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ProductsBack/IHC-plus(tm) Sprouty 4 / SPRY4 Antibody

IHC-plus(tm) Sprouty 4 / SPRY4 Antibody

LS-B15881
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSPRY4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) Sprouty 4 / SPRY4 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    The predicted MW is 11kDa/32kDa, while the observed MW by Western blot was 37kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 11kDa/32kDa, while the observed MW by Western blot was 37kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81848
  • Target name
    SPRY4
  • Target description
    sprouty RTK signaling antagonist 4
  • Target synonyms
    HH17, protein sprouty homolog 4, sprouty homolog 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sprouty 4/Spry-4/SPRY4 antigen affinity purified polyclonal antibody (Catalog # A04343-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sprouty 4/Spry-4/SPRY4 at approximately 35KD. The expected band size for Sprouty 4/Spry-4/SPRY4 is at 35KD.
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