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ProductsBack/IHC-plus(tm) STK33 Antibody (clone 3F10)

IHC-plus(tm) STK33 Antibody (clone 3F10)

LS-B4535
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSTK33
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) STK33 Antibody (clone 3F10)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control Jurkat. Immunofluorescence (10 ug/ml) - positive control HeLa cells.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF (10 µg/ml), IHC, IHC-P (10 µg/ml), WB Western Blot (Cell lysate) - positive control Jurkat. Immunofluorescence (10 ug/ml) - positive control HeLa cells.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F10
  • Conjugate
    Unconjugated
  • Gene ID65975
  • Target name
    STK33
  • Target description
    serine/threonine kinase 33
  • Target synonyms
    SPGF93, serine/threonine-protein kinase 33
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STK33 using anti-STK33 antibody (M07375). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK33 antigen affinity purified monoclonal antibody (M07375) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STK33 at approximately 58 kDa. The expected band size for STK33 is at 58, 50 kDa.
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