IHC-plus(tm) TGM2 / Transglutaminase 2 Antibody

LifeSpan BioSciences

IHC-plus(tm) TGM2 / Transglutaminase 2 Antibody

23

Immunohistochemistry: LS-B2235 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2235 was determined to be 5 ug/ml. Immunoprecipitation (Native verified): 10 ug/mg protein lysate. Use Protein A. Western Blot: 5-10 ug/ml for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma.. IHC, IHC-P (5 µg/ml), IP, WB (5 - 10 µg/ml) Immunohistochemistry: LS-B2235 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2235 was determined to be 5 ug/ml. Immunoprecipitation (Native verified): 10 ug/mg protein lysate. Use Protein A. Western Blot: 5-10 ug/ml for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

TGM2

transglutaminase 2

G(h), TG(C), TGC, hTG2, tTG, protein-glutamine gamma-glutamyltransferase 2, C polypeptide, protein-glutamine-gamma-glutamyltransferase, TGase C, TGase H, TGase II, TGase-2, erythrocyte transglutaminase, heart G alpha(h), hhG alpha(h), isopeptidase TGM2, protein G alpha(h), protein-glutamine deamidase TGM2, protein-glutamine dopaminyltransferase TGM2, protein-glutamine histaminyltransferase TGM2, protein-glutamine noradrenalinyltransferase TGM2, protein-glutamine serotonyltransferase TGM2, tTgase, tissue transglutaminase, transglutaminase C, transglutaminase H, transglutaminase II

Rabbit

IgG

Human, Mouse, Rat

-20°C,2°C to 8°C

41116161