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ProductsBack/IHC-plus(tm) TIMP2 Antibody (aa178-193)

IHC-plus(tm) TIMP2 Antibody (aa178-193)

LS-B8328
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
TargetTIMP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TIMP2 Antibody (aa178-193)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 5 ug/ml (PLP fixed tissue), 1 ug/ml (Frozen tissues).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-Fr, IHC-P (10 µg/ml), WB Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 5 ug/ml (PLP fixed tissue), 1 ug/ml (Frozen tissues).
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7077
  • Target name
    TIMP2
  • Target description
    TIMP metallopeptidase inhibitor 2
  • Target synonyms
    CSC-21K, DDC8, metalloproteinase inhibitor 2, testicular secretory protein Li 57, tissue inhibitor of metalloproteinases 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Bovine, Canine, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIMP2 using anti-TIMP2 antibody (PB9830). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Spleen Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Mouse Thymus Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP2 antigen affinity purified polyclonal antibody (Catalog # PB9830) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP2 at approximately 24 kDa. The expected band size for TIMP2 is at 24 kDa.
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