IHC-plus(tm) TLR4 Antibody (clone 1E11)

LifeSpan BioSciences

IHC-plus(tm) TLR4 Antibody (clone 1E11)

23

Immunohistochemistry: LS-B2254 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2254 was determined to be 5 ug/ml.Flow Cytometry: 3ug/10e6 cellsOptimal dilutions to be determined by researcher.. ELISA (1:2000 - 1:4000), Flo, IHC, IHC-P (5 µg/ml) Immunohistochemistry: LS-B2254 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2254 was determined to be 5 ug/ml.Flow Cytometry: 3ug/10e6 cellsOptimal dilutions to be determined by researcher.

Flow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1E11

0.5 mg/ml

Unconjugated

TLR4

toll like receptor 4

ARMD10, CD284, TLR-4, TOLL, toll-like receptor 4, hToll, homolog of Drosophila toll, toll like receptor 4 protein

Mouse

IgG2a

Human

-20°C,2°C to 8°C

41116161