IHC-plus(tm) TNFSF10 / TRAIL Antibody

LifeSpan BioSciences

IHC-plus(tm) TNFSF10 / TRAIL Antibody

23

Immunohistochemistry: LS-B7420 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTRAIL/Apo2L (10 ng/ml), a concentration of 150 ng/ml of this antibody is required. ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTRAIL/Apo2L (LS-C104497) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7420 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTRAIL/Apo2L (10 ng/ml), a concentration of 150 ng/ml of this antibody is required. ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTRAIL/Apo2L (LS-C104497) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

TNFSF10

TNF superfamily member 10

APO2L, Apo-2L, CD253, TANCR, TL2, TNLG6A, TRAIL, tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis inducing ligand TRAIL, chemokine tumor necrosis factor ligand superfamily member 10, tumor necrosis factor (ligand) family, member 10, tumor necrosis factor (ligand) superfamily, member 10, tumor necrosis factor ligand 6A, tumor necrosis factor superfamily member 10

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

41116161