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ProductsBack/IHC-plus(tm) TNNI2 Antibody (clone 11E6)

IHC-plus(tm) TNNI2 Antibody (clone 11E6)

LS-B4963
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTNNI2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TNNI2 Antibody (clone 11E6)
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11E6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7136
  • Target name
    TNNI2
  • Target description
    troponin I2, fast skeletal type
  • Target synonyms
    AMCD2B, DA2B, DA2B1, FSSV, fsTnI, troponin I, fast skeletal muscle, troponin I fast twitch 2, troponin I type 2 (skeletal, fast), troponin I, skeletal, fast
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI2 antigen affinity purified polyclonal antibody (Catalog # A07355-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI2 at approximately 24KD. The expected band size for TNNI2 is at 21KD.
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Immunohistochemistry analysis in human skeletal muscle and testis tissues using HPA055938 antibody. Corresponding TNNI2 RNA-seq data are presented for the same tissues.
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