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ProductsBack/IHC-plus(tm) TSG101 Antibody (clone 5B7)

IHC-plus(tm) TSG101 Antibody (clone 5B7)

LS-B5547
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTSG101
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TSG101 Antibody (clone 5B7)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.. ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000) Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7251
  • Target name
    TSG101
  • Target description
    tumor susceptibility 101
  • Target synonyms
    TSG10, VPS23, tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, tumor susceptibility gene 10, tumor susceptibility gene 101, tumor susceptibility protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (A01233-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Hek293 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (Catalog # A01233-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44KD. The expected band size for TSG101 is at 44KD.
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