More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) ULK3 Antibody (aa445-458)

IHC-plus(tm) ULK3 Antibody (aa445-458)

LS-B14654
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetULK3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ULK3 Antibody (aa445-458)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 47kD band in Rat Brain and Eye lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.6kD according to Mouse NP_001092906.1 and Rat XP_001077085.2. The 47kD band was successfully blocked by incubation with the immunizing peptide.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:64000) Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 47kD band in Rat Brain and Eye lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.6kD according to Mouse NP_001092906.1 and Rat XP_001077085.2. The 47kD band was successfully blocked by incubation with the immunizing peptide.
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25989
  • Target name
    ULK3
  • Target description
    unc-51 like kinase 3
  • Target synonyms
    serine/threonine-protein kinase ULK3
  • Host
    Goat
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of 293 cells using ULK3 Polyclonal Antibody
Product group Antibodies
Cusabio
ULK3 AntibodyCSB-PA004389
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetULK3
  • SizePrice
Product group Antibodies
Bioss
ULK3 Recombinant Antibody, AbBy Fluor-594 ConjugatedBSM-61618R-BF594
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetULK3
  • SizePrice
Product group Antibodies
Assay Genie
ULK3 Monoclonal AntibodyCAB5959
ReactivityHuman
TargetULK3
  • SizePrice
Anti-ULK3 Antibody
Product group Antibodies
Antibodies.com
Anti-ULK3 AntibodyA85002
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
MedChem Express
ULK3 Antibody (YA2355, Rabbit)HY-P82610
TargetULK3
  • SizePrice
Product group Antibodies
References
Everest Biotech
Goat anti-ULK3 (aa445-458)EB11184
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Porcine
TargetULK3
  • SizePrice
Immunohistochemical staining of human colon shows distinct cytoplasmic positivity with a granular pattern in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-ULK3 AntibodyHPA040474
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetULK3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ULK3 Antibody (Internal)LS-C358883
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetULK3
  • SizePrice
Figure 1. Western blot analysis of ULK3 using anti-ULK3 antibody (PB9519). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Human Placenta Tissue Lysate at 50ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK3 antigen affinity purified polyclonal antibody (Catalog # PB9519) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK3 at approximately 53 kDa. The expected band size for ULK3 is at 53 kDa.
Product group Antibodies
Boster Bio
Anti-ULK3 Antibody Picoband(r)PB9519-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetULK3
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ULK3 (C-term) Antibody102-20107
ApplicationsWestern Blot
TargetULK3
  • SizePrice