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IHC-plus(tm) UQCRFS1 Antibody (aa181-209)

LS-B15601
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetUQCRFS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) UQCRFS1 Antibody (aa181-209)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC, IHC-P (1:10 - 1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7386
  • Target name
    UQCRFS1
  • Target description
    ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
  • Target synonyms
    MC3DN10, RIP1, RIS1, RISP, UQCR5, cytochrome b-c1 complex subunit Rieske, mitochondrial, Rieske iron-sulfur protein, Rieske protein UQCRFS1, complex III subunit 5, cytochrome b-c1 complex subunit 5, epididymis secretory sperm binding protein, ubiquinol-cytochrome c reductase iron-sulfur subunit
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UQCRFS1 using anti-UQCRFS1 antibody (M08781-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRFS1 antigen affinity purified monoclonal antibody (Catalog # M08781-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRFS1 at approximately 23 kDa. The expected band size for UQCRFS1 is at 30 kDa.
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