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UQCRFS1 Antibody

CAB6784
Assay Genie
TargetUQCRFS1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    UQCRFS1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7386
  • Target name
    UQCRFS1
  • Target description
    ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
  • Target synonyms
    complex III subunit 5; cytochrome b-c1 complex subunit 5; cytochrome b-c1 complex subunit Rieske, mitochondrial; epididymis secretory sperm binding protein; MC3DN10; Rieske iron-sulfur protein; Rieske protein UQCRFS1; RIP1; RIS1; RISP; ubiquinol-cytochrome c reductase iron-sulfur subunit; UQCR5
  • Host
    Rabbit
  • Protein IDP47985
  • Protein Name
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UQCRFS1 using anti-UQCRFS1 antibody (M08781-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRFS1 antigen affinity purified monoclonal antibody (Catalog # M08781-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRFS1 at approximately 23 kDa. The expected band size for UQCRFS1 is at 30 kDa.
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