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ProductsBack/IHC-plus(tm) VCL / Vinculin Antibody

IHC-plus(tm) VCL / Vinculin Antibody

LS-B10133
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVCL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) VCL / Vinculin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Vinculin antibody can be used for detection of Vinculin by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 117 kDa Observed: 120kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) Vinculin antibody can be used for detection of Vinculin by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 117 kDa Observed: 120kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7414
  • Target name
    VCL
  • Target description
    vinculin
  • Target synonyms
    CMD1W, CMH15, HEL114, MV, MVCL, VINC, vinculin, epididymis luminal protein 114, epididymis secretory sperm binding protein, meta-vinculin, metavinculin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VCL using anti-VCL antibody (A01207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human SGC-7901 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
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