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ProductsBack/IHC-plus(tm) WNT2B Antibody (Internal)

IHC-plus(tm) WNT2B Antibody (Internal)

LS-A8572
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
TargetWNT2B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) WNT2B Antibody (Internal)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.. IHC, IHC-P (5 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7482
  • Target name
    WNT2B
  • Target description
    Wnt family member 2B
  • Target synonyms
    WNT13, protein Wnt-2b, XWNT2, Xenopus, homolog of, wingless-type MMTV integration site family, member 13, wingless-type MMTV integration site family, member 2B
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Wnt2b using anti-Wnt2b antibody (A04879-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human SKOV3 whole cell lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse Neuro-2a whole cell lysates, Lane 10: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # A04879-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD.
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