IHC-plus(tm) WNT5A Antibody (clone 3D10)

LifeSpan BioSciences

IHC-plus(tm) WNT5A Antibody (clone 3D10)

23

Immunohistochemistry: LS-B3878 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3878 was determined to be 1:200. ELISA (1:10000), IHC, IHC-P (1:200 - 1:400), WB (1:100 - 1:500) Immunohistochemistry: LS-B3878 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3878 was determined to be 1:200

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

3D10

Unconjugated

WNT5A

Wnt family member 5A

hWNT5A, protein Wnt-5a, WNT-5A protein, epididymis secretory sperm binding protein, wingless-type MMTV integration site family, member 5A

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161