IHC-plus(tm) WNT5A Antibody (clone 6F2)

LifeSpan BioSciences

IHC-plus(tm) WNT5A Antibody (clone 6F2)

23

Immunohistochemistry: LS-B3859 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3859 was determined to be 1:200.. ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:400), WB (1:100 - 1:500) Immunohistochemistry: LS-B3859 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3859 was determined to be 1:200.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

6F2

Unconjugated

WNT5A

Wnt family member 5A

hWNT5A, protein Wnt-5a, WNT-5A protein, epididymis secretory sperm binding protein, wingless-type MMTV integration site family, member 5A

Mouse

IgG1

Human

-20°C,2°C to 8°C

41116161