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IHC-plus(tm) ZBTB7A / Pokemon Antibody

LS-B6522
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse, Opossum, Rat
TargetZBTB7A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ZBTB7A / Pokemon Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51341
  • Target name
    ZBTB7A
  • Target description
    zinc finger and BTB domain containing 7A
  • Target synonyms
    FBI-1, FBI1, LRF, MNDLFH, TIP21, ZBTB7, ZNF857A, pokemon, zinc finger and BTB domain-containing protein 7A, HIV-1 1st-binding protein 1, HIV-1 inducer of short transcripts binding protein, POK erythroid myeloid ontogenic factor, POZ and Krueppel erythroid myeloid ontogenic factor, TTF-I-interacting peptide 21, factor binding IST protein 1, factor that binds to inducer of short transcripts protein 1, leukemia/lymphoma-related factor, lymphoma related factor, pokemon 1, zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein, zinc finger protein 857A
  • Host
    Rabbit
  • Reactivity
    Chicken, Human, Mouse, Opossum, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9910) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 72 kDa. The expected band size for ZBTB7A is at 61 kDa.
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