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ProductsBack/IHC-plus(tm) ZIC3 Antibody (aa178-189)

IHC-plus(tm) ZIC3 Antibody (aa178-189)

LS-B9538
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat
TargetZIC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ZIC3 Antibody (aa178-189)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 70kD band in Human, Muse and Rat Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kD according to NP_003404.1. The 70kD band was successfully blocked by incubation with the immunizing peptide.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:64000) Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 70kD band in Human, Muse and Rat Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kD according to NP_003404.1. The 70kD band was successfully blocked by incubation with the immunizing peptide.
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7547
  • Target name
    ZIC3
  • Target description
    Zic family zinc finger 3
  • Target synonyms
    HTX, HTX1, VACTERLX, ZNF203, zinc finger protein ZIC 3, Zic family member 3 (odd-paired homolog, Drosophila), heterotaxy 1, zinc finger protein 203, zinc finger protein of the cerebellum 3
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ZIC3 using anti-ZIC3 antibody (A04050-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC3 antigen affinity purified polyclonal antibody (Catalog # A04050-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC3 at approximately 56-60KD. The expected band size for ZIC3 is at 56-60KD.
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