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ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

IKB alpha (phospho Ser32/Ser36) antibody

GTX32224
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNFKBIA
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Overview

  • Supplier
    GeneTex
  • Product Name
    IKB alpha (phospho Ser32/Ser36) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4792
  • Target name
    NFKBIA
  • Target description
    NFKB inhibitor alpha
  • Target synonyms
    EDAID2; IkappaBalpha; I-kappa-B-alpha; IKBA; ikB-alpha; MAD-3; major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25963
  • Protein Name
    NF-kappa-B inhibitor alpha
  • Scientific Description
    This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Downregulation of Hypoxia-Inducible Factor-1alpha by RNA Interference Alleviates the Development of Collagen-Induced Arthritis in Rats. Hu Y et al., 2020 Mar 6, Mol Ther Nucleic Acids
    Read more
  • Au Clusters Treat Rheumatoid Arthritis with Uniquely Reversing Cartilage/Bone Destruction. Gao F et al., 2019 Apr 3, Adv Sci (Weinh)
    Read more

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Figure 1. Western blot analysis of IKB Alpha using anti-IKB Alpha antibody (PB9291). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB Alpha antigen affinity purified polyclonal antibody (Catalog # PB9291) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB Alpha at approximately 39KD. The expected band size for IKB Alpha is at 36KD.
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