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Western Blot analysis of Hela cells using Helios Polyclonal Antibody
Western Blot analysis of Hela cells using Helios Polyclonal Antibody
Western Blot analysis of Hela cells using Helios Polyclonal Antibody

IKZF2 Antibody

CSB-PA008257
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIKZF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    IKZF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22807
  • Target name
    IKZF2
  • Target description
    IKAROS family zinc finger 2
  • Target synonyms
    ANF1A2, HELIOS, ICHAD, IMDIA, ZNF1A2, ZNFN1A2, zinc finger protein Helios, ikaros family zinc finger protein 2, zinc finger DNA binding protein Helios, zinc finger protein, subfamily 1A, 2 (Helios)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKS7
  • Protein Name
    Zinc finger protein Helios
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IKZF2 using anti-IKZF2 antibody (A05228-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF2 antigen affinity purified polyclonal antibody (Catalog # A05228-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKZF2 at approximately 70 kDa. The expected band size for IKZF2 is at 60 kDa.
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