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ProductsBack/IKZF2 / HELIOS Antibody

IKZF2 / HELIOS Antibody

LS-C832336
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIKZF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IKZF2 / HELIOS Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.26 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID22807
  • Target name
    IKZF2
  • Target description
    IKAROS family zinc finger 2
  • Target synonyms
    ANF1A2, HELIOS, ICHAD, IMDIA, ZNF1A2, ZNFN1A2, zinc finger protein Helios, ikaros family zinc finger protein 2, zinc finger DNA binding protein Helios, zinc finger protein, subfamily 1A, 2 (Helios)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IKZF2 using anti-IKZF2 antibody (A05228-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF2 antigen affinity purified polyclonal antibody (Catalog # A05228-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKZF2 at approximately 70 kDa. The expected band size for IKZF2 is at 60 kDa.
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