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Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

IL33 Polyclonal Antibody

BS-2633R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIl33
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Overview

  • Supplier
    Bioss
  • Product Name
    IL-33 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID77125
  • Target name
    Il33
  • Target description
    interleukin 33
  • Target synonyms
    9230117N10Rik, Il-33, Il1f11, NF-HEV, interleukin-33, nuclear factor from high endothelial venules
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8BVZ5
  • Protein Name
    Interleukin-33
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Effects of 1,25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model. Chen B et al., 2017 Jun, Int Immunopharmacol
    Read more

Datasheet

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FAQ

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