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ProductsBack/IL33 antibody [Carly-1-4]

IL33 antibody [Carly-1-4]

GTX33930
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIl33
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL33 antibody [Carly-1-4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Western blot: 1:1000
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Carly-1-4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID77125
  • Target name
    Il33
  • Target description
    interleukin 33
  • Target synonyms
    9230117N10Rik, Il-33, Il1f11, NF-HEV, interleukin-33, nuclear factor from high endothelial venules
  • Host
    Human
  • Isotype
    IgG2
  • Protein IDQ8BVZ5
  • Protein Name
    Interleukin-33
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free). Methods: IL-33 (mouse) was coated on an ELISA plate at 1microg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40microg/ml with a twofold serial dilution) together with 100microl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).
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Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1).Methods: IL-33 (mouse) was coated on an ELISA plate at 1microg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40microg/ml with a twofold serial dilution) together with 100microl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).
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IL33 antibody [Bondy-1-1]GTX33928
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Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 31KD. The expected band size for IL33 is at 31KD.
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Anti-IL33 Antibody Picoband(r)A00113-1-CARRIER-FREE
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Western blot analysis using anti-IL-33 (mouse), pAb (Prod. No. AG-25A-0047) at 1:2000 dilution. 1: Mouse IL-33 (His-tagged). 2: Human IL-33 (His-tagged). 3: Unrelated protein (His-tagged) (negative control).
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Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
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