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ProductsBack/Interleukin 33 (IL33) Polyclonal Antibody

Interleukin 33 (IL33) Polyclonal Antibody

CAU24925
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetIl33
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Overview

  • Supplier
    Biomatik
  • Product Name
    Interleukin 33 (IL33) Polyclonal An
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID77125
  • Target name
    Il33
  • Target description
    interleukin 33
  • Target synonyms
    9230117N10Rik; Il-; Il1f; Il1f11; Il-33; interleukin-33; NF-H; NF-HEV; nuclear factor from high endothelial venules
  • Host
    Rabbit
  • Protein IDQ8BVZ5
  • Protein Name
    Interleukin-33
  • Scientific Description
    The Interleukin 33 (IL33) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
Product group Antibodies
References
Bioss Antibodies
IL33 Polyclonal AntibodyBS-2633R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetIl33
  • SizePrice
Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 31KD. The expected band size for IL33 is at 31KD.
Product group Antibodies
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Anti-IL33 Antibody Picoband(r)A00113-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetIl33
  • SizePrice
Western blot analysis using anti-IL-33 (mouse), pAb (Prod. No. AG-25A-0047) at 1:2000 dilution. 1: Mouse IL-33 (His-tagged). 2: Human IL-33 (His-tagged). 3: Unrelated protein (His-tagged) (negative control).
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AdipoGen Life Sciences
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TargetIl33
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IL33 antibody [Carly-1-4]GTX33930
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TargetIl33
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