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ProductsBack/IL17E antibody [12V5]

IL17E antibody [12V5]

GTX52830
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetIL25
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL17E antibody [12V5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12V5
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID64806
  • Target name
    IL25
  • Target description
    interleukin 25
  • Target synonyms
    IL17E; interleukin-17E; interleukin-25
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H293
  • Protein Name
    Interleukin-25
  • Scientific Description
    The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17E using anti-IL17E antibody (A04429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL25 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17E antigen affinity purified polyclonal antibody (Catalog # A04429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17E at approximately 17KD. The expected band size for IL17E is at 17KD.
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