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IP analysis of HeLa cell lysate using GTX32675 IL18 antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000
IP analysis of HeLa cell lysate using GTX32675 IL18 antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000
IP analysis of HeLa cell lysate using GTX32675 IL18 antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000

IL18 antibody

GTX32675
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL18
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL18 antibody - KO/KD Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IP: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3606
  • Target name
    IL18
  • Target description
    interleukin 18
  • Target synonyms
    IGIF, IL-18, IL-1g, IL1F4, interleukin-18, IFN-gamma-inducing factor, IL-1 gamma, iboctadekin, interleukin 18 (interferon-gamma-inducing factor), interleukin-1 gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14116
  • Protein Name
    Interleukin-18
  • Scientific Description
    The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Aberrant expression and localization of the RAP1 shelterin protein contribute to age-related phenotypes.
    Read more
  • Gasdermin D pore structure reveals preferential release of mature interleukin-1. Xia S et al., 2021 May, Nature
    Read more
  • Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA. Zhang C et al., 2021 May, Mol Med Rep
    Read more
  • Low shear stress induced vascular endothelial cell pyroptosis by TET2/SDHB/ROS pathway. Chen J et al., 2021 Jan, Free Radic Biol Med
    Read more

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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9006) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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